例如："lncRNA", "apoptosis", "WRKY"

Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.

Nat. Struct. Mol. Biol.2014 Dec;21(12):1075-81. Epub 2014 Nov 02
Peng Ge 1 , Zeynep A Oztug Durer 2 , Dmitri Kudryashov 3 , Z Hong Zhou 4 , Emil Reisler 5
Peng Ge 1 , Zeynep A Oztug Durer 2 , Dmitri Kudryashov 3 , Z Hong Zhou 4 , Emil Reisler 5

[No authors listed]

Author information
  • 1 1] Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA. [2] California NanoSystems Institute (CNSI), UCLA, Los Angeles, California, USA.
  • 2 Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, USA.
  • 3 Department of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio, USA.
  • 4 1] Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA. [2] California NanoSystems Institute (CNSI), UCLA, Los Angeles, California, USA. [3] Molecular Biology Institute, UCLA, Los Angeles, California, USA.
  • 5 1] Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, USA. [2] Molecular Biology Institute, UCLA, Los Angeles, California, USA.
全文

摘要

Essential cellular processes involving the actin cytoskeleton are regulated by auxiliary proteins that can sense the nucleotide state of actin. Here we report cryo-EM structures for ADP-bound and ADP-beryllium fluoride (ADP-BeFx, an ADP-Pi mimic)-bound actin filaments in complex with the β-propeller domain of yeast coronin 1 (crn1), at 8.6-Å resolution. Our structures reveal the main differences in the interaction of coronin with the two nucleotide states of F-actin. We derived pseudoatomic models by fitting the atomic structures of actin and coronin into the EM envelopes and confirmed the identified interfaces on actin by chemical cross-linking, fluorescence spectroscopy and actin mutagenesis. The models offer a structural explanation for the nucleotide-dependent effects of coronin on cofilin-assisted remodeling of F-actin.

图表

基因

CRN1

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