Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B.

As a newly discovered member of the HSP70 family, heat shock protein A12B is involved in brain ischemic injury. According to our previous study, microRNA-134 (miR-134) could target by binding to its 3'-untranslated region (UTR). However, the regulation of miR-134 on Hduanyu184212B and their role in protecting neuronal cells from ischemic injury are unclear. In this study, the miR-134 expression level was manipulated, and the Hduanyu184212B protein levels were also determined in oxygen-glucose deprivation (OGD)-treated primary cultured neuronal cells in vitro and mouse brain after middle cerebral artery occlusion (MCAO)-induced ischemic stroke in vivo. The results showed that miR-134 expression levels increased in primary cultured neuronal cells and mouse brain from 12h to 7 day reoxygenation/reperfusion after 1h OGD or 1h MCAO treatment. miR-134 overexpression promoted neuronal cell death and apoptosis by decreasing Hduanyu184212B protein levels. Conversely, downregulating miR-134 reduced neuronal cell death and apoptosis by enhancing Hduanyu184212B protein levels. Also, Hduanyu184212B siRNA could block miR-134 inhibitor-mediated neuroprotection against OGD-induced neuronal cell injury in vitro. Taken together, miR-134 might influence neuronal cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by negatively modulating Hduanyu184212B protein expression in a posttranscriptional manner.

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