Upregulated expression of SSTR1 is involved in neuronal apoptosis and is coupled to the reduction of bcl-2 following intracerebral hemorrhage in adult rats.

Somatostatins are peptide hormones that regulate diverse cellular processes, such as neurotransmission, cell proliferation, apoptosis, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. is a member of the superfamily of somatostatin receptors possessing seven-transmembrane segments. Aberrant expression of duanyu1942R1 has been implicated in several human diseases, including pseudotumor cerebri, and oncogenic osteomalacia. In this study, we investigated a potential role of duanyu1942R1 in the regulation of neuronal apoptosis in the course of intracerebral hemorrhage (ICH). A rat ICH model in the caudate putamen was established and subjected to behavioral tests. Western blot and immunohistochemistry indicated a remarkable up-regulation of duanyu1942R1 expression surrounding the hematoma after ICH. Double-labeled immunofluorescence showed that duanyu1942R1 was mostly co-localized with neurons, and was rarely distributed in activated astrocytes and microglia. Additionally, duanyu1942R1 co-localized with active-caspase-3 and bcl-2 around the hematoma. The expression of active-caspase-3 was parallel with that of duanyu1942R1 in a time-dependent manner. In addition, duanyu1942R1 knockdown specifically resulted in reduced neuronal apoptosis in PC12 cells. All our findings suggested that up-regulated duanyu1942R1 contributed to neuronal apoptosis after ICH, which was accompanied with reduced expression of bcl-2.

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