例如:"lncRNA", "apoptosis", "WRKY"

Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly.

Cell Rep. 2014 Jul 10;8(1):103-13. Epub 2014 Jun 19
Yukiko Murota 1 , Hirotsugu Ishizu 2 , Shinichi Nakagawa 3 , Yuka W Iwasaki 4 , Shinsuke Shibata 5 , Miharu K Kamatani 4 , Kuniaki Saito 4 , Hideyuki Okano 5 , Haruhiko Siomi 4 , Mikiko C Siomi 6
Yukiko Murota 1 , Hirotsugu Ishizu 2 , Shinichi Nakagawa 3 , Yuka W Iwasaki 4 , Shinsuke Shibata 5 , Miharu K Kamatani 4 , Kuniaki Saito 4 , Hideyuki Okano 5 , Haruhiko Siomi 4 , Mikiko C Siomi 6
+ et al

[No authors listed]

Author information
  • 1 Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan; Department of Molecular Biology, Keio University School of Medicine, Tokyo 160-8582, Japan.
  • 2 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, Japan.
  • 3 RIKEN, Wako, Saitama 351-0198, Japan.
  • 4 Department of Molecular Biology, Keio University School of Medicine, Tokyo 160-8582, Japan.
  • 5 Department of Biomedical Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
  • 6 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, Japan. Electronic address: siomim@bs.s.u-tokyo.ac.jp.

摘要


PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable for formation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
cell typeclip-antibodysource name
Yb-CLIP from OSC
Drosophila melanogaster GSM1325469: Yb-CLIP from OSC; Drosophila melanogaster; RNA-Seq RNA-Seq Illumina HiSeq 2000 Ovarian Somatic Cells (OSC) Yb-antibody (generated in this study) Ovarian Somatic Cells (OSC)