例如:"lncRNA", "apoptosis", "WRKY"

The rhino-deadlock-cutoff complex licenses noncanonical transcription of dual-strand piRNA clusters in Drosophila.

Cell. 2014 Jun 05;157(6):1364-1379
Fabio Mohn 1 , Grzegorz Sienski 2 , Dominik Handler 2 , Julius Brennecke 3
Fabio Mohn 1 , Grzegorz Sienski 2 , Dominik Handler 2 , Julius Brennecke 3

[No authors listed]

Author information
  • 1 Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohrgasse 3, 1030 Vienna, Austria. Electronic address: fabio.mohn@imba.oeaw.ac.at.
  • 2 Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohrgasse 3, 1030 Vienna, Austria.
  • 3 Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohrgasse 3, 1030 Vienna, Austria. Electronic address: julius.brennecke@imba.oeaw.ac.at.

摘要


Argonaute proteins of the PIWI clade are central to transposon silencing in animal gonads. Their target specificity is defined by 23-30 nt PIWI interacting RNAs (piRNAs), which mostly originate from discrete genomic loci termed piRNA clusters. Here, we show that a complex composed of Rhino, Deadlock, and Cutoff (RDC) defines dual-strand piRNA clusters genome-wide in Drosophila ovaries. The RDC is anchored to H3K9me3-marked chromatin in part via Rhino's chromodomain. Depletion of Piwi results in loss of the RDC and small RNAs at a subset of piRNA clusters, demonstrating a feedback loop between Piwi and piRNA source loci. Intriguingly, profiles of RNA polymerase II occupancy, nascent transcription, and steady-state RNA levels reveal that the RDC licenses noncanonical transcription of dual-strand piRNA clusters. Likely, this process involves 5' end protection of nascent RNAs and suppression of transcription termination. Our data provide key insight into the regulation and evolution of piRNA clusters.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
chip antibodychip antibody vendordevelopment stagegermline knock-downmolecule subtypesexsource nametissue
ribosomal RNA depleted total RNA from control germline knock-down ovaries, replicate 1
Drosophila melanogaster GSM1346535: ribosomal RNA depleted total RNA from control germline knock-down ovaries, replicate 1; Drosophila melanogaster; RNA-Seq RNA-Seq Illumina HiSeq 2000 4-6 days old adult control female ribosomal RNA depleted total RNA from control germline knock-down ovaries ovary
5'-capped RNA from control germline knock-down ovaries
Drosophila melanogaster GSM1346542: 5'-capped RNA from control germline knock-down ovaries; Drosophila melanogaster; RNA-Seq RNA-Seq Illumina HiSeq 2000 4-6 days old adult control female 5'-capped RNA from control germline knock-down ovaries ovary
nascent RNA from global nuclear run on (GRO) in rhino germline knock-down ovaries
Drosophila melanogaster GSM1346541: nascent RNA from global nuclear run on (GRO) in rhino germline knock-down ovaries; Drosophila melanogaster; RNA-Seq RNA-Seq Illumina HiSeq 2000 4-6 days old adult rhino female nascent RNA from global nuclear run on (GRO) in rhino germline knock-down ovaries ovary
nascent RNA from global nuclear run on (GRO) in control germline knock-down ovaries
Drosophila melanogaster GSM1346540: nascent RNA from global nuclear run on (GRO) in control germline knock-down ovaries; Drosophila melanogaster; RNA-Seq RNA-Seq Illumina HiSeq 2000 4-6 days old adult control female nascent RNA from global nuclear run on (GRO) in control germline knock-down ovaries ovary
small RNAs from a Piwi-IP in piwi germline knock-down ovaries (=somatic piRNAs)
Drosophila melanogaster GSM1346534: small RNAs from a Piwi-IP in piwi germline knock-down ovaries (=somatic piRNAs); Drosophila melanogaster; RNA-Seq RNA-Seq Illumina HiSeq 2000 4-6 days old adult piwi small RNA female small RNAs from a Piwi-IP in piwi germline knock-down ovaries (=somatic piRNAs) ovary