例如:"lncRNA", "apoptosis", "WRKY"

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development.

Plant Physiol.2014 May 7;165(3):1203-1220. Epub 2014 May 7
Mary Prathiba Joseph 1 , Csaba Papdi 1 , László Kozma-Bognár 1 , István Nagy 1 , Marta López-Carbonell 1 , Gábor Rigó 1 , Csaba Koncz 1 , László Szabados 2
Mary Prathiba Joseph 1 , Csaba Papdi 1 , László Kozma-Bognár 1 , István Nagy 1 , Marta López-Carbonell 1 , Gábor Rigó 1 , Csaba Koncz 1 , László Szabados 2
+ et al

[No authors listed]

Author information
  • 1 Institute of Plant Biology, Biological Research Centre, H-6726 Szeged, Hungary (M.P.J., C.P., L.K.-B., I.N., G.R., C.K., L.S.);Department of Plant Biology, University of Barcelona, 08028 Barcelona, Spain (M.L.-C.);Max-Planck-Institut für Züchtungsforschung, 50829 Cologne, Germany (C.K.); andRoyal Holloway, University of London, Surrey TW20 0EX, United Kingdom (C.P.).
  • 2 Institute of Plant Biology, Biological Research Centre, H-6726 Szeged, Hungary (M.P.J., C.P., L.K.-B., I.N., G.R., C.K., L.S.);Department of Plant Biology, University of Barcelona, 08028 Barcelona, Spain (M.L.-C.);Max-Planck-Institut für Züchtungsforschung, 50829 Cologne, Germany (C.K.); andRoyal Holloway, University of London, Surrey TW20 0EX, United Kingdom (C.P.) szabados@brc.hu.

摘要


Seed germination is controlled by environmental signals, including light and endogenous phytohormones. Abscisic acid (ABA) inhibits, whereas gibberellin promotes, germination and early seedling development, respectively. Here, we report that ZFP3, a nuclear C2H2 zinc finger protein, acts as a negative regulator of ABA suppression of seed germination in Arabidopsis (Arabidopsis thaliana). Accordingly, regulated overexpression of ZFP3 and the closely related ZFP1, ZFP4, ZFP6, and ZFP7 zinc finger factors confers ABA insensitivity to seed germination, while the zfp3 zfp4 double mutant displays enhanced ABA susceptibility. Reduced expression of several ABA-induced genes, such as RESPONSIVE TO ABSCISIC ACID18 and transcription factor ABSCISIC ACID-INSENSITIVE4 (ABI4), in ZFP3 overexpression seedlings suggests that ZFP3 negatively regulates ABA signaling. Analysis of ZFP3 overexpression plants revealed multiple phenotypic alterations, such as semidwarf growth habit, defects in fertility, and enhanced sensitivity of hypocotyl elongation to red but not to far-red or blue light. Analysis of genetic interactions with phytochrome and abi mutants indicates that ZFP3 enhances red light signaling by photoreceptors other than phytochrome A and additively increases ABA insensitivity conferred by the abi2, abi4, and abi5 mutations. These data support the conclusion that ZFP3 and the related ZFP subfamily of zinc finger factors regulate light and ABA responses during germination and early seedling development.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
genotypesource nametissue
ZFP3ox/ABA
Arabidopsis thaliana GSM1183039: ZFP3ox/ABA; Arabidopsis thaliana; RNA-Seq RNA-Seq AB 5500xl Genetic Analyzer ZFP3 overexpressing ABA-treated seedlings, 84 hours seedling
ZFP3ox/control
Arabidopsis thaliana GSM1183038: ZFP3ox/control; Arabidopsis thaliana; RNA-Seq RNA-Seq AB 5500xl Genetic Analyzer ZFP3 overexpressing control seedlings, 36 hours seedling
Col-0/ABA
Arabidopsis thaliana GSM1183037: Col-0/ABA; Arabidopsis thaliana; RNA-Seq RNA-Seq AB 5500xl Genetic Analyzer Col-0 wild type ABA-treated seedlings, 84 hours seedling
Col-0/control
Arabidopsis thaliana GSM1183036: Col-0/control; Arabidopsis thaliana; RNA-Seq RNA-Seq AB 5500xl Genetic Analyzer Col-0 wild type control seedlings, 36 hours seedling