[No authors listed]
The profile of microRNAs (miRNAs) altered following middle cerebral artery occlusion (MCAO) and miRNAs are involved in angiogenesis following cerebral ischemia. miRâ376bâ5p was decreased following MCAO, however, whether miRâ376bâ5p is important in angiogenesis remains to be elucidated. The present study was designed to identify whether miRâ376bâ5p is involved in angiogenesis following cerebral ischemia and to elucidate the underlying mechanisms. A rat MCAO model was established and quantitative polymerase chain reaction was performed to analyze the mRNA expression level of miRâ376bâ5p for 1 to 7 days. In addition, the density of microvessels and the relative mRNA and protein levels of hypoxiaâinducible factorâ1 α (HIFâ1α), vascular endothelial growth factor A (VEGFA) and Notch1 were measured. The miRâ376bâ5p mimic or the miRâ376bâ5p inhibitor were transfected into hypoxic human umbilical vein endothelial cells (HUVECs), and the proliferation, migration and tube formation were measured. To further examine the underlying mechanisms, shRNA was transfected into cells to knock down HIFâ1α, and angiogenesis and the expression of associated molecules, including HIFâ1α, VEGFA and Notch1 were compared between each group. Our results demonstrated that miRâ376bâ5p repressed angiogenesis in vivo and in vitro, and miRâ376bâ5p inhibited angiogenesis in HUVECs by targeting the HIFâ1αâmediated VEGFA/Notch1 signaling pathway. These findings provide new insights into angiogenesis therapy for cerebral ischemia.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
{{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
{{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} |
{{ list.authorName }} {{ list.authorName }} |