Overexpression of NOTCH-regulated ankyrin repeat protein is associated with breast cancer cell proliferation.

BACKGROUND/AIM:NOTCH-regulated ankyrin repeat protein has been implicated in crosstalk between NOTCH and wingless-type mouse mammary tumor virus integration site (WNT) signals during development. Our study aimed to clarify its role in breast cancer cells. MATERIALS AND METHODS:Public microarray data were used to analyze gene expression in human and rat breast cancer. A short interfering RNA was introduced into MCF7 and T47D human breast cancer cells for silencing. Gene expression was analyzed by quantitative polymerase chain reaction. RESULTS:The NRduanyu37 transcript was commonly overexpressed in various rat mammary cancer models. In addition, a subset of human breast cancer also expressed high levels of NRduanyu37 transcript, which correlated positively with up-regulation of cell proliferation-related genes. Silencing of NRduanyu37 suppressed the growth of MCF7 and T47D cells and lowered the expression of cell cycle-related genes in MCF7 may stimulate cell proliferation in human breast cancer.

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