例如:"lncRNA", "apoptosis", "WRKY"

Let-7c inhibits metastatic ability of mouse hepatocarcinoma cells via targeting mannoside acetylglucosaminyltransferase 4 isoenzyme A.

Int. J. Biochem. Cell Biol.2014 Aug;53:1-8. Epub 2014 Apr 24
Yanjie Guo 1 , Sheng Li 1 , Jianhua Qu 1 , Lin Ye 2 , Shujing Wang 1 , Jianhui Fan 1 , Qi Wang 3 , Jianing Zhang 4
Yanjie Guo 1 , Sheng Li 1 , Jianhua Qu 1 , Lin Ye 2 , Shujing Wang 1 , Jianhui Fan 1 , Qi Wang 3 , Jianing Zhang 4
+ et al

[No authors listed]

Author information
  • 1 Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China.
  • 2 Department of Oncology, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
  • 3 Department of Respiratory Medicine, The Second Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
  • 4 Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China; School of Life Science and Medicine, Dalian University of Technology, 2 Linggong Road, Dalian 116024, China. Electronic address: jnzhang@dlmedu.edu.cn.

摘要


Aberrant glycosylation may promote tumor invasion and metastasis. To investigate whether microRNA (miRNA) is involved in glycosylation-related metastasis, we examined the role of let-7c, a well-known tumor-suppressor miRNA, in glycosylation in murine hepatocarcinoma cell lines Hca-F and Hca-P. We found that let-7c level was higher in Hca-P cells (with lower lymphatic metastasis potential) than in Hca-F cells (with higher lymphatic metastasis potential). Overexpression of let-7c decreased hyper-N-glycosylation of Hca-F cells and repressed their metastatic and invasive ability. Mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) is a key glycosyltransferase in the pathway of synthesizing complex N-glycans. Bioinformatics analysis indicates that Mgat4a may be a target of let-7c, which has been verified by dual-luciferase reporter gene assay. Furthermore, the anti-metastatic effect of overexpressed let-7c is similar to that of Mgat4a siRNAs transfection. Hence, our results suggest that let-7c may inhibit the metastatic ability of Hca-F cells, at least partially, via repressing Mgat4a activity.

KEYWORDS: Glycosyl-transferase, Let-7c, Mgat4a, MicroRNA, N-glycosylation