[Production and properties evaluation of monoclonal antibodies against Pseudomonas aeruginosa exotoxin A].

AIM:Production, study of properties and evaluation of a possibility to use monoclonal antibodies against Pseudomonas aeruginosa exotoxin A (ETA) for the detection of molecules of recombinant exotoxin A and anatoxin. MATERIALS AND METHODS:Producer hybridomas for monoclonal antibodies against ETA were generated. Recombinant exotoxin A, its atoxic forms, P. aeruginosa anatoxin and P. aeruginosa PA-103 live culture were used for immunization of mice. RESULTS:The experiment of fusion of malignant cell line with immune lymphocytes obtained from a mouse immunized with recombinant ETA turned out to be the most productive. Intensity of interaction of monoclonal antibodies with recombinant atoxic forms of ETA was evaluated in enzyme immunoassay. Protective (toxin-neutralizing) activity of antibodies in cell culture and the ability to detect exotoxin A in latex-agglutination reaction were studied. Antibodies of hybrid culture No. 21 are able to detect molecules of recombinant ETA and anatoxin in the latex-agglutination reaction. CONCLUSION:Use of the antibodies produced for testing toxigenicity of P. aeruginosa strains as well as detection of anatoxin in the technological process of production of prophylaxis preparations based on exotoxin A is expected.

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