例如:"lncRNA", "apoptosis", "WRKY"

CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.

Biochim. Biophys. Acta. 2014 Jul;1842(7):1121-9. Epub 2014 Apr 13
Chang Li 1 , Lei Wang 1 , Jing Zhang 2 , Mi Huang 1 , Fulton Wong 3 , Xuexue Liu 1 , Fei Liu 1 , Xiukun Cui 1 , Guohua Yang 4 , Jiaxiang Chen 1 , Ying Liu 1 , Jiuxiang Wang 1 , Shengjie Liao 1 , Meng Gao 1 , Xuebin Hu 1 , Xinhua Shu 5 , Qing Wang 1 , Zhan Yin 6 , Zhaohui Tang 7 , Mugen Liu 8
Chang Li 1 , Lei Wang 1 , Jing Zhang 2 , Mi Huang 1 , Fulton Wong 3 , Xuexue Liu 1 , Fei Liu 1 , Xiukun Cui 1 , Guohua Yang 4 , Jiaxiang Chen 1 , Ying Liu 1 , Jiuxiang Wang 1 , Shengjie Liao 1 , Meng Gao 1 , Xuebin Hu 1 , Xinhua Shu 5 , Qing Wang 1 , Zhan Yin 6 , Zhaohui Tang 7 , Mugen Liu 8
+ et al

[No authors listed]

Author information
  • 1 Key Laboratory of Molecular Biophysics of the Ministry of Education, Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
  • 2 Key Laboratory of Molecular Biophysics of the Ministry of Education, Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Cellular and Molecular Immunology, Institute of Immunology, Medical College of Henan University, Kaifeng, Henan 475004, PR China.
  • 3 Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA; Department of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.
  • 4 Department of Medical Genetics, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
  • 5 MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, Edinburgh, UK.
  • 6 Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, PR China.
  • 7 Key Laboratory of Molecular Biophysics of the Ministry of Education, Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. Electronic address: zh_tang@163.com.
  • 8 Key Laboratory of Molecular Biophysics of the Ministry of Education, Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. Electronic address: lium@mail.hust.edu.cn.

摘要


Mutations in the ceramide kinase-like gene (CERKL) are associated with severe retinal degeneration. However, the exact function of the encoded protein (CERKL) remains unknown. Here we show that CERKL interacts with mitochondrial thioredoxin 2 (TRX2) and maintains TRX2 in the reduced redox state. Overexpression of CERKL protects cells from apoptosis under oxidative stress, whereas suppressing CERKL renders cells more sensitive to oxidative stress. In zebrafish, CERKL protein prominently locates in the outer segment and inner segment of the photoreceptor of the retina. Knockdown of CERKL in the zebrafish leads to an increase of retinal cell death, including cone and rod photoreceptor degeneration. Signs of oxidative damage to macromolecules were also detected in CERKL deficient zebrafish retina. Our results show that CERKL interacts with TRX2 and plays a novel key role in the regulation of the TRX2 antioxidant pathway and, for the first time, provides an explanation of how mutations in CERKL may lead to retinal cell death.

KEYWORDS: Apoptosis, CERKL, Oxidative stress, Retina, TRX2