Rapid adaptive optical recovery of optimal resolution over large volumes.
Nat. Methods. 2014 Jun;11(6):625-8. Epub 2014 Apr 13
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摘要
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
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文献解读
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