[No authors listed]
BACKGROUND:Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from fore brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days. RESULTS:In PNS, the 10 up (â)- or down (â)-regulated proteins exhibiting the largest morphine-induced change were selected, excised manually from the gel and identified by MALDI-TOF MS/MS: 1-(gi|148747414, Guanine deaminase), â2.5Ã; 2-(gi|17105370, Vacuolar-type proton ATP subunit B, brain isoform), â2.6Ã; 3-(gi|1352384, Protein disulfide-isomerase A3), â3.4Ã; 4-(gi|40254595, Dihydropyrimidinase-related protein 2), â3.6Ã; 5-(gi|149054470, N-ethylmaleimide sensitive fusion protein, isoform CRAa), â2.0Ã; 6-(gi|42476181, Malate dehydrogenase, mitochondrial precursor), â1.4Ã; 7-(gi|62653546, Glyceraldehyde-3-phosphate dehydrogenase), â1.6Ã; 8-(gi|202837, Aldolase A), â1.3Ã; 9-(gi|31542401, Creatine kinase B-type), â0.86Ã; 10-(gi|40538860, Aconitate hydratase, mitochondrial precursor), â1.3Ã. The identified proteins were of cytoplasmic (1, 4, 5, 7, 9), cell membrane (2), endoplasmic reticulum (3) and mitochondrial (6, 8, 10) origin and 9 of them were significantly increased, 1.3-3.6Ã. The 4 out of 9 up-regulated proteins (4, 6, 7, 10) were described as functionally related to oxidative stress; the 2 proteins participate in genesis of apoptotic cell death.In PM, the 18 up (â)- or down (â)-regulated proteins were identified by LC-MS/MS and were of plasma membrane [Brain acid soluble protein, â2.1Ã; trimeric Gβ subunit, â2.0x], myelin membrane [MBP, â2.5Ã], cytoplasmic [Internexin, â5.2Ã; DPYL2, â4.9Ã; Ubiquitin hydrolase, â2.0Ã; 60S ribosomal protein, â2.7Ã; KCRB, â2.6Ã; Sirtuin-2, â2.5Ã; Peroxiredoxin-2, â2.2Ã; Septin-11, â2.2Ã; TERA, â2.1Ã; SYUA, â2.0Ã; Coronin-1A, â5.4Ã] and mitochondrial [Glutamate dehydrogenase 1, â2.7Ã; SCOT1, â2.2Ã; Prohibitin, â2.2Ã; Aspartate aminotransferase, â2.2Ã] origin. Surprisingly, the immunoblot analysis of the same PM resolved by 2D-ELFO indicated that the "active", morphine-induced pool of Gβ subunits represented just a minor fraction of the total signal of Gβ which was decreased 1.2x only. The dominant signal of Gβ was unchanged. CONCLUSION:Brain cortex of rats exposed to increasing doses of morphine is far from being adapted. Significant up-regulation of proteins functionally related to oxidative stress and apoptosis suggests a major change of energy metabolism resulting in the state of severe brain cell "discomfort" or even death.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
{{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
{{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} |
{{ list.authorName }} {{ list.authorName }} |