[Relationship of reduced lung function with Th1/Th2 polarization, STAT4/6 expression in rats of chronic obstructive pulmonary disease].

OBJECTIVE:To study the correlations between lung function and Th1/Th2 polarization, signal transducer and activator of transcription proteins in rats of chronic obstructive pulmonary disease (COPD). METHODS:Sixty rats were randomly divided into normal control group, sham group and model group. The COPD rat models were developed by lipopolysaccharide (LPS). The changes of pulmonary function were detected at 28 d after modeling. The lung tissues of rats were observed by HE staining, and serum IFN-γ, IL-4, IL-12, and IL-13 were determined by ELISA. The mRNA expressions of IFN-γ and IL-4 were examined by PCR, and proteins in lung tissues were detected by Western blotting. RESULTS:Inflammation of lung tissues was more serious, the levels of serum IFN-γ, IL-12 and Th1/Th2, IFN-γ mRNA and protein in lung tissues were higher, and lung function, the levels of serum IL-4 and IL-13, IL-4 mRNA and duanyu18136 protein in lung tissues were lower in model group than those in normal control and sham groups (P < 0.05 or P < 0.01). Correlation analysis showed that there were negative correlations between pulmonary function parameters and IFN-γ, Th1/Th2, duanyu18134 protein, and positive correlations between pulmonary function parameters and IL-4, IL-13, IL-4 mRNA, duanyu18136 protein (P < 0.01 or P < 0.05). CONCLUSION:The reduced lung function was found related to airway inflammation, cell Th1/Th2 imbalance in COPD. duanyu18134 and duanyu18136 involve in the regulation of Th1/Th2 cells. All of the above factors lead to the occurrence of COPD in rats.

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