Two-color in situ hybridization of whole-mount mouse embryos.
Methods Mol Biol. 2014;1092:17-30. doi:10.1007/978-1-60327-292-6_2
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摘要
RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.
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基因功能
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原始数据
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文献解读
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