A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development.
Environ. Toxicol. Chem.2014 Jan;33(1):238-42. doi:10.1002/etc.2428. Epub 2013 Nov 20
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摘要
Monitoring expression of the developmentally regulated genes shh, sox2, and tnnt1 by reverse transcriptase polymerase chain reaction (RT-PCR) allows determination of the rate of embryogenesis in zebrafish (Danio rerio) embryos without direct visual observation. The utility of combining this approach and morphological methods during toxicity studies was demonstrated with embryos developing at either 28.5â°C or 24.5â°C and with embryos exposed to sublethal doses of silver nanoparticles.
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基因功能
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原始数据
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文献解读
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