Molecular characterization, alternative splicing and expression analysis of bovine DBC1.

Deleted in breast cancer 1 (DBC1, KIAA1967, p30 DBC) is a novel protein that has been recently shown to bind and regulate SIRT1. Loss of function of DBC1 increased SIRT1 deacetylase activity, which promotes "browning" of WAT by deacetylating peroxisome proliferator-activated receptor (PPARγ) on Lys268 and Lys293. In the present study, we have cloned and characterized the bovine DBC1 gene. Two transcript variants of bovine DBC1 were identified, designated DBC1-A and DBC1-B, respectively, which were both located in nucleus. Protein sequence analysis showed that DBC1-A was well conserved across species. Expression analysis of DBC1 in seven different tissues of calves and bulls by RT-PCR indicated that the two transcripts were ubiquitously expressed. However, the relatively level of DBC1-A was higher when compared to DBC1-B in all examined tissues. Surprisingly, the expression of DBC1-A was extraordinary high in calves adipose tissue, which implicated its potential key role in regulating calve adipocyte development. These findings provide new insight into our understanding of the biochemical characteristics and physiological role of bovine DBC1.

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