[No authors listed]
Cellular pyrin domain-only protein 1 (cPOP1) is a pyrin domain (PYD)-containing protein that can regulate inflammation by preventing the assembly of inflammasome via direct interaction with ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain). In this study, cPOP1, corresponding to amino acids 1-87, was overexpressed in Escherichia coli using an engineered C-terminal polyhistidine tag. cPOP1 was then purified to homogeneity and crystallized at 293â K. Finally, X-ray diffraction data were collected to a resolution of 3.6â à from a crystal belonging to the cubic space group P2â3 with unit-cell parameters a=b=c=94.12â à , α=β=γ=90.00°.
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