Src kinases mediate the interaction of the apical determinant Bazooka/PAR3 with STAT92E and increase signalling efficiency in Drosophila ectodermal cells.

Intercellular communication depends on the correct organization of the signal transduction complexes. In many signalling pathways, the mechanisms controlling the overall cell polarity also localize components of these pathways to different domains of the plasma membrane. In the Drosophila ectoderm, the pathway components are highly polarized with apical localization of the receptor, the associated kinase and the protein itself. The apical localization of duanyu181392E is independent of the receptor complex and is due to its direct association with the apical determining protein Bazooka (Baz). Here, we find that interaction depends on the presence of the Drosophila Src kinases. In the absence of Src, duanyu181392E cannot bind to Baz in cells or in whole embryos, and this correlates with an impairment of JAK/duanyu1813 signalling function. We believe that the requirement of Src proteins for duanyu181392E apical localization is mediated through Baz, as we can co-precipitate Src with Baz but not with This is the first time that a functional link between cell polarity, the JAK/duanyu1813 signalling pathway and the Src kinases has been established in a whole organism.

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