Expression and characterization of the nitrile reductase queF from E. coli.
Enzyme Microb. Technol.2013 Mar 5;52(3):129-33. Epub 2012 Dec 14
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摘要
The expression and characterization of a nitrile reductase from Escherichia coli K-12 (EcoNR), a newly discovered enzyme class, is described. This enzyme has a potential application for an alternative nitrile reduction pathway. The enzyme activity towards its natural substrate, preQ(0), was demonstrated and optimal working conditions were found to be at 37°C and at pH 7 with Tris buffer.
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基因功能
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原始数据
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文献解读
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