例如:"lncRNA", "apoptosis", "WRKY"

A role for the RNA-binding protein MOS2 in microRNA maturation in Arabidopsis.

Cell Res.2013 May;23(5):645-57. Epub 2013 Feb 12
Xueying Wu 1 , Yupeng Shi , Jingrui Li , Le Xu , Yuda Fang , Xin Li , Yijun Qi
Xueying Wu 1 , Yupeng Shi , Jingrui Li , Le Xu , Yuda Fang , Xin Li , Yijun Qi
+ et al

[No authors listed]

Author information
  • 1 College of Life Sciences, Beijing Normal University, Beijing 100875, China.

摘要


microRNAs (miRNAs) play important roles in the regulation of gene expression. In Arabidopsis, mature miRNAs are processed from primary miRNA transcripts (pri-miRNAs) by nuclear HYL1/SE/DCL1 complexes that form Dicing bodies (D-bodies). Here we report that an RNA-binding protein MOS2 binds to pri-miRNAs and is involved in efficient processing of pri-miRNAs. MOS2 does not interact with HYL1, SE, and DCL1 and is not localized in D-bodies. Interestingly, in the absence of MOS2, the recruitment of pri-miRNAs by HYL1 is greatly reduced and the localization of HYL1 in D-bodies is compromised. These data suggest that MOS2 promotes pri-miRNA processing through facilitating the recruitment of pri-miRNAs by the Dicing complexes.

基因


原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
ecotypegenotypesource nametissue
mos2-2 rep2
Arabidopsis thaliana GSM1025550: mos2-2 rep2; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina Genome Analyzer IIx Col-0 mos2-2 seedlings 14 day old seedlings
mos2-2 rep1
Arabidopsis thaliana GSM1025549: mos2-2 rep1; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina Genome Analyzer IIx Col-0 mos2-2 seedlings 14 day old seedlings
Col-0 rep2
Arabidopsis thaliana GSM1025548: Col-0 rep2; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina Genome Analyzer IIx Col-0 wild type seedlings 14 day old seedlings
Col-0 rep1
Arabidopsis thaliana GSM1025547: Col-0 rep1; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina Genome Analyzer IIx Col-0 wild type seedlings 14 day old seedlings