Silencing of the ARP2/3 complex disturbs pancreatic cancer cell migration.

BACKGROUND:Actin-related protein 2/3 complex is an actin nucleator responsible for actin cytoskeleton branching which is essential for efficient cell migration. MATERIALS AND METHODS:The expression of the seven complex subunits was assessed in pancreatic cancer cell lines and in normal pancreatic samples by quantitative RT-PCR. siRNA-mediated silencing was used to study the contribution of each duanyu372/3 complex member to pancreatic cancer cell and were the most highly expressed complex members, while and were expressed at low levels. Silencing of the duanyu372/3 complex subunits typically resulted in reduced cell migration capacity. In particular, silencing of duanyu37C4 significantly reduced cell migration in all studied cell lines, with a major impact on Hs700T and HPAFII migration (50% and 68% decrease, p<0.001). CONCLUSION:We offer comprehensive expression data on the duanyu372/3 complex members for pancreatic cancer and normal pancreas. In addition, we show cell line-specific differences in duanyu372/3 complex subunit dependency on cell migratory potential, and suggest duanyu37C4 to be one of the key members of the duanyu372/3 complex in pancreatic cancer.

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