[No authors listed]
Systematic analysis of gene overexpression phenotypes provides an insight into gene function, enzyme targets, and biological pathways. Here, we describe a novel functional genomics platform that enables a highly parallel and systematic assessment of overexpression phenotypes in pooled cultures. First, we constructed a genome-level collection of ~5100 yeast barcoder strains, each of which carries a unique barcode, enabling pooled fitness assays with a barcode microarray or sequencing readout. Second, we constructed a yeast open reading frame (ORF) galactose-induced overexpression array by generating a genome-wide set of yeast transformants, each of which carries an individual plasmid-born and sequence-verified ORF derived from the Saccharomyces cerevisiae full-length EXpression-ready (FLEX) collection. We combined these collections genetically using synthetic genetic array methodology, generating ~5100 strains, each of which is barcoded and overexpresses a specific ORF, a set we termed "barFLEX." Additional synthetic genetic array allows the barFLEX collection to be moved into different genetic backgrounds. As a proof-of-principle, we describe the properties of the barFLEX overexpression collection and its application in synthetic dosage lethality studies under different environmental conditions.
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YDR182W-A, NFS1, BUD3, PMP1, ABP1, ALR2, MMM1, PML1, POM34, MEF1, GIS3, YLR126C, NOP56, MSS51, IFH1, ECM22, NDL1, CWC24, RPS29A, MFG1, DUN1, PMT1, MTF2, RTK1, PSF1, RPS11A, RPC11, MAK21, TPS2, YDR090C, UBC1, CBS2, SNU56, MET32, RMD5, MTH1, HDA2, PEP7, PEX3, MSN5, LCD1, PLM2, SDH7, FDC1, PRE7, MRPL16, MCM2, ALK2, RCR1, PDX3, RDH54, RAD16, YSY6, PBP2, SAE2, XRN1, RCK1, GPI10, NUP145, MRH4, DUO1, SDS23, ERG4, STF2, YGR012W, SNU71, SMD1, RRP46, PBP1, AMA1, PEX21, PUP2, HUA1, EFG1, BIO2, ASG7, QCR8, PBS2, SIP4, APS3, RAD26, HUL4, MGM101, MCR1, CMC1, HAP4, ALY1, DID2, BET3, PPM2, SPO21, PSH1, RTS1, MSA1, LSC1, RPB2, PET123, MSB1, PUS7, TMA16, NDD1, SHQ1, NEO1, PRM2, MPH1, LYS1, TCB3, ZDS2, ITT1, MFT1, YML020W, AIM34, PLB2, RCO1, YTA12, YMR155W, GYL1, RNT1, GTO3, ZDS1, GOT1, PHA2, CUS2, UBP10, NPR1, YNL181W, IMP4, BOP3, NRM1, YNR048W, BIO4, PUS1, YIG1, HHO1, SVL3, TAF3, YPR015C, RLF2, YPR063C, YLH47, GOS1, SPO11, YHL009W-A, RPL14B, ARG4, BRL1, VMA10, ERG7, KSP1, RTC3, RPC10, YHR177W, YHR210C, TIR1, FIR1, IES5, SPR6, RSP5, YER187W
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