Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae.
[No authors listed]
摘要
Kar9p is required for correct positioning of the mitotic spindle in Saccharomyces cerevisiae. The in vivo function of Kar9p is well understood, but no structural information is available. Additionally, molecular details of how Kar9p interacts with other proteins are scarce. Full-length Kar9p was expressed in Escherichia coli, purified and crystallized. Diffraction data were collected and processed at 7â à resolution. One crystal showed diffraction to 3â à resolution. The crystals that diffracted to 7â à resolution belonged to space group P3, with unit-cell parameters a = b = 195.02, c = 257.15â à , α = β = 90, γ = 120°. The crystal that diffracted to 3â à resolution belonged to space group P222, with unit-cell parameters a = 46.37, b = 74.64, c = 133.63â à , α = β = γ = 90°.
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基因功能
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原始数据
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文献解读
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