Blood vessel epicardial substance (Bves) regulates epidermal tight junction integrity through atypical protein kinase C.

Bves is widely observed in the cell junction of the skin, epicardium, intestine, and cornea of both developmental embryos and mature adults. However, it is not clear how Bves confers its role in intercellular adhesion. Here, we identified the zebrafish bves (zBves) and found that the epidermal barrier function could be disrupted after knockdown of Bves, and these zBves morphants were sensitive to osmotic stress. A loss of zBves would affect the partitioning defective protein (PAR) junctional complex identified by the rescue experiment with tjp-2/ZO-2 or the PAR complex (par-3, par-6, and prkci/atypical mRNAs, in which the survival rate of embryos increased 11, 24, 25, and 28%, respectively, after injection with junctional components; the tjp-2 and mRNA-rescued embryos also had 24 and 45% decreases in the defective rate. Immunofluorescent studies demonstrated that the aggregation of aduanyu1531 around the cell junctions had disintegrated in zBves morphants. However, the expression and assembly of zBves were not influenced by These results indicate that a loss of zBves affects the proteins involved in the pathway of the PAR junctional complex, especially and both aduanyu1531 and Bves are indispensable to claudin expression.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}