Transcriptional regulation of human novel gene SPATA12 promoter by AP-1 and HSF.

Human is a spermatogenesis associated gene and is supposed to function as an inhibitor during male germ cell development. duanyu1842TA12 is specifically expressed in spermatocytes, spermatids, and spermatozoa of human testis. In order to understand the regulation mechanism of duanyu1842TA12 gene expression, we identified and characterized the duanyu1842TA12 gene core promoter region and transcription factor binding sites by using reporter gene assays. AP-1 is founded to be a potential transcriptional activator of The promoter activity of duanyu1842TA12 was drastically declined after AP-1 binding site mutation or deletion. We also demonstrated that AP-1 combined with Smad3/4 contributes to the transcriptional regulation of duanyu1842TA12 in response to TGF-β1. The expression of duanyu1842TA12 could be induced by TGF-β1 in a dose-dependent manner, suggesting that AP-1 as an activator plays a role in the regulation of duanyu1842TA12 promoter. We have also shown that heat shock treatment could activate the expression of duanyu1842TA12 and transcription factor HSF binding sites in the duanyu1842TA12 promoter might be responsible for this heat-induction. These results suggested that AP-1 and HSF may play an important role in regulating duanyu1842TA12 promoter activity.

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