Formation of a heterooctameric complex between aspartate α-decarboxylase and its cognate activating factor, PanZ, is CoA-dependent.
Biochem. Biophys. Res. Commun.2012 Sep 28;426(3):350-5. Epub 2012 Aug 24
Diana C F Monteiro 1
,
Michael D Rugen
,
Dale Shepherd
,
Shingo Nozaki
,
Hironori Niki
,
Diana C F Monteiro 1
,
Michael D Rugen
,
Dale Shepherd
,
Shingo Nozaki
,
Hironori Niki
,
Michael E Webb
+ et al
摘要
The existence of a fifth essential protein for pantothenate biosynthesis in some enteric bacteria has recently been reported by Stuecker et al. [10] and Nozaki et al. (in press) [9]. This protein, PanZ, catalyses the activation of the PanD zymogen to form ADC and is essential for prototrophic growth. In this paper, we characterise the interaction of PanZ with coenzyme A and a constitutively inactive mutant of PanD using a combination of isothermal titration calorimetry and mass spectrometry. These approaches reveal that the two proteins interact with nanomolar affinity in a CoA-dependent fashion to form a heterooctameric complex.
