Production of mouse pups from germline transmission-failed knockout chimeras.
Transgenic Res.2013 Feb;22(1):195-200. doi:10.1007/s11248-012-9635-x. Epub 2012 Jul 24
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摘要
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
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基因功能
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原始数据
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文献解读
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