例如:"lncRNA", "apoptosis", "WRKY"

Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2.

Int. J. Mol. Med.2012 Oct;30(4):884-8. doi:10.3892/ijmm.2012.1065. Epub 2012 Jul 16
Ying Zheng 1 , Shudong Sheng , Haiyan Wang , Xiaoqing Jia , Yanqiu Hu , Yayun Qian , Yongze Zhu , Jianjun Wang
Ying Zheng 1 , Shudong Sheng , Haiyan Wang , Xiaoqing Jia , Yanqiu Hu , Yayun Qian , Yongze Zhu , Jianjun Wang
+ et al

[No authors listed]

Author information
  • 1 Department of Histology and Embryology, Medical College, Yangzhou University, Yangzhou 225001, PR China.

摘要


Pold2 is a subunit of the DNA polymerase δ complex, encoding a protein involved in DNA replication and repair. In this study, using a yeast two-hybrid screening technique and the common cDNA fragment of the mouse PIAS2 as a bait, Pold2 was found to interact with PIAS2. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid. In vivo evidence of Pold2 association with PIAS2 was obtained by co-immunoprecipitation using HEK-293 cells. Subcellular localization studies demonstrated that Pold2 and PIAS2 were partially co-localized in mammalian cells. Collectively, our results suggest that Pold2 interacts under physiological conditions with PIAS2.