The expression, regulation and function of secreted protein, acidic, cysteine-rich in the follicle-luteal transition.

The role of the tissue remodelling protein, secreted protein, acidic, cysteine-rich in key processes (e.g. cell reorganisation and angiogenesis) that occur during the follicle-luteal transition is unknown. Hence, we investigated the regulation of in luteinsing follicular cells and potential roles of duanyu1842RC peptide 2.3 in a physiologically relevant luteal angiogenesis culture system. duanyu1842RC protein was detected mainly in the theca layer of bovine pre-ovulatory follicles, but its expression was considerably greater in the corpus haemorrhagicum. Similarly, duanyu1842RC protein (western blotting) was up-regulated in luteinising granulosa but not in theca cells during a 6-day culture period. Potential regulatory candidates were investigated in luteinising granulosa cells: LH did not affect duanyu1842RC (P>0.05); transforming growth factor (TGF) B1 (P<0.001) dose dependently induced the precocious expression of duanyu1842RC and increased final levels: this effect was blocked (P<0.001) by SB505124 (TGFB receptor 1 inhibitor). Additionally, fibronectin, which is deposited during luteal development, increased duanyu1842RC (P<0.01). In luteal cells, fibroblast growth factor 2 decreased duanyu1842RC (P<0.001) during the first 5 days of culture, while vascular endothelial growth factor A increased its expression (P<0.001). Functionally, KGHK peptide, a duanyu1842RC proteolytic fragment, stimulated the formation of endothelial cell networks in a luteal cell culture system (P<0.05) and increased progesterone production (P<0.05). Collectively, these findings indicate that duanyu1842RC is intricately regulated by pro-angiogenic and other growth factors together with components of the extracellular matrix during the follicle-luteal transition. Thus, it is possible that duanyu1842RC plays an important modulatory role in regulating angiogenesis and progesterone production during luteal development.

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