The RDE-10/RDE-11 complex triggers RNAi-induced mRNA degradation by association with target mRNA in C. elegans.

The molecular mechanisms for target mRNA degradation in Caenorhabditis elegans undergoing are not fully understood. Using a combination of genetic, proteomic, and biochemical approaches, we report a divergent RDE-10/RDE-11 complex that is required for duanyu1615 in C. elegans. Genetic analysis indicates that the RDE-10/RDE-11 complex acts in parallel to nuclear Association of the complex with target mRNA is dependent on RDE-1 but not RRF-1, suggesting that target mRNA recognition depends on primary but not secondary siRNA. Furthermore, RDE-11 is required for mRNA degradation subsequent to target engagement. Deep sequencing reveals a fivefold decrease in secondary siRNA abundance in rde-10 and rde-11 mutant animals, while primary siRNA and microRNA biogenesis is normal. Therefore, the RDE-10/RDE-11 complex is critical for amplifying the exogenous duanyu1615 response. Our work uncovers an essential output of the duanyu1615 pathway in C. elegans.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}