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Synaptic localisation of agmatinase in rat cerebral cortex revealed by virtual pre-embedding.

Amino Acids. 2012 Sep;43(3):1399-403. doi:10.1007/s00726-011-1195-5. Epub 2011 Dec 13
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摘要


Light microscopic evidence suggested a synaptic role for agmatinase, an enzyme capable of inactivating the putative neurotransmitter and endogenous anti-depressant agmatine. Using electron microscopy and an alternative pre-embedding approach referred to as virtual pre-embedding, agmatinase was localised pre- and postsynaptically, to dendritic spines, spine and non-spine terminals, and dendritic profiles. In dendritic spines, labelling displayed a tendency towards the postsynaptic density. These results further strengthen a synaptic role for agmatine and strongly suggest a regulatory role for synaptically expressed agmatinase.

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