[No authors listed]
We conducted a mitotic localization study on gene products encoded by 56 uncharacterized fission yeast ORFs that were transcriptionally up-regulated during meiotic division. Despite meiotic gene induction, these genes were expressed during mitosis as well. Seven gene products were localized in the nucleus and/or chromatin; another one was a mitosis-specific spindle pole body component and, intriguingly, its human homologue was also localized in the centrosome of cultured HeLa cells. Two products appeared to be localized in cytoplasmic microtubules, whereas four were mitochondrial proteins. Three other proteins were found in the medial ring upon cytokinesis and another was localized on the entire cell periphery. The remaining 38 proteins were detected in the cytoplasm and showed varied spatial patterns. This systematic study helps our integrated understanding of all the protein functions in the fission yeast as a eukaryotic model.
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SPCC1442.02, mug174, SPCC553.01c, SPCC1739.04c, SPCC1322.02, SPCC63.05, SPCC4F11.03c, SPCC663.15c, SPBC1539.02, mug184, SPBC115.02c, mug100, SPBC32H8.09, SPBC31F10.17c, mug110, SPBC27B12.05, mug164, SPBC28E12.04, uds1, SPBC23G7.06c, SPBC25H2.15, SPBC21D10.08c, SPBC211.03c, SPBC713.09, SPBC19C2.10, ppk22, mug166, SPBC651.12c, ell1, spo7, SPAC7D4.13c, SPAP27G11.12, SPAC25A8.02, mug165, SPAC17G8.12, ftp105, SPAC17A2.08c, mug74, SPAC1952.17c, mug125, mug105, ppk3, mcp1, mug99, mug129, SPAC144.07c, SPAC12G12.09, SPAC12B10.10, SPAC323.03c, tbc1, SPAPB21F2.02, SPAC8C9.04, ppk13, SPAC890.04c, ppk16, SPAC3H8.04
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