NM_001013649.3 gene is down-regulated in human colorectal adenocarcinoma.

The present study aimed to evaluate the expression of NM_001013649.3 in human colorectal adenocarcinoma tissue and its correlation with clinicopathological aspects of the disease. Through the combination of a cDNA subtractive library and cDNA microarray analysis, a differentially expressed sequence tag ES274081 was identified whose full-length cDNA sequence (NM_001013649.3) was obtained through BLASTn analysis on NCBI. Reverse transcription quantitative real-time PCR was used to assess the expression levels of NM_001013649.3 in cancer and adjacent normal colorectal tissues from 30 patients with colorectal adenocarcinoma. The mRNA levels of NM_001013649.3 were significantly lower in the cancer tissues compared to the adjacent normal colorectal tissues of 25 out of 30 (83%) patients (p<0.005). Furthermore, the rate of down-regulation was significantly higher in moderately differentiated samples (21 out of 23; 91%) compared to poorly differentiated samples (3 out of 6; 50%) (p<0.05). The mRNA levels of NM_001013649.3 are significantly down-regulated in colorectal adenocarcinoma tissue, and this down-regulation is most likely correlated with the differentiation status of the cancer, indicating that NM_001013649.3 is potentially involved in the pathogenesis of colorectal adenocarcinoma.

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