[Relationship between activation of mannan-binding lectin complement and NF-kappaB in diabetic nephropathy].

OBJECTIVE:To study the activation of mannose-binding lectin (MBL) complement in STZ-induced diabetic nephropathy (DN) rats and its relationship with NF-kappaB. METHODS:Sprague-Dawley (SD) rats were randomly divided into two groups: normal control and diabetic nephropathy. Diabetes was induced by intraperitoneal injection of STZ. Five rats were sacrificed at the end of week 1, 2, 4, 8 respectively. Blood glucose, 24 h urine, 24 h urinary albumin, serum creatinine (Scr), body mass and kidney mass were examined at the same time points respectively. Creatinine clearance and renal hypertrophy index were calculated. The renal expression of MBL, membrane attack complex (MAC) and NF-kappaB were determined by immunohistochemistry. RESULT:MBL, MAC and NF-kappaB expression were significantly increased in glomerulus of diabetic nephropathy rats compared to the controls. The expression of MBL was positively correlated with NF-kappaB expression. CONCLUSION:The activation of mannose-binding lectin complement participates in the onset and development of DN.

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