例如:"lncRNA", "apoptosis", "WRKY"

The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements.

Nat. Cell Biol.2011 Aug 21;13(9):1029-39
Constance Richter 1 , Katarzyna Oktaba , Jonas Steinmann , Jürg Müller , Juergen A Knoblich
Constance Richter 1 , Katarzyna Oktaba , Jonas Steinmann , Jürg Müller , Juergen A Knoblich

[No authors listed]

Author information
  • 1 Institute of Molecular Biotechnology of the Austrian Academy of Science, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
全文

摘要


In Drosophila, defects in asymmetric cell division often result in the formation of stem-cell-derived tumours. Here, we show that very similar terminal brain tumour phenotypes arise through a fundamentally different mechanism. We demonstrate that brain tumours in l(3)mbt mutants originate from overproliferation of neuroepithelial cells in the optic lobes caused by derepression of target genes in the Salvador-Warts-Hippo (SWH) pathway. We use ChIP-sequencing to identify L(3)mbt binding sites and show that L(3)mbt binds to chromatin insulator elements. Mutating l(3)mbt or inhibiting expression of the insulator protein gene mod(mdg4) results in upregulation of SWH pathway reporters. As l(3)mbt tumours are rescued by mutations in bantam or yorkie or by overexpression of Expanded, the deregulation of SWH pathway target genes is an essential step in brain tumour formation. Therefore, very different primary defects result in the formation of brain tumours, which behave quite similarly in their advanced stages.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
chip antibodysource namestraintemperaturetissue
Input DNA, Canton S
Drosophila melanogaster GSM722526: Input DNA, Canton S ChIP-Seq Illumina Genome Analyzer II none Third instar larval brain and wing/haltere/third leg imaginal discs tissue, input Canton S 25C third instar larval brain and wing/haltere/third leg imaginal discs
Input DNA, w1118
Drosophila melanogaster GSM722525: Input DNA, w1118 ChIP-Seq Illumina Genome Analyzer II none Third instar larval brain and wing/haltere/third leg imaginal discs tissue, input w1118 25C third instar larval brain and wing/haltere/third leg imaginal discs
l(3)mbt ChIP, Canton S
Drosophila melanogaster GSM722524: l(3)mbt ChIP, Canton S ChIP-Seq Illumina Genome Analyzer II guinea pig 1 anti-l(3)mbt 1244-1477aa (glycine elution) Third instar larval brain and wing/haltere/third leg imaginal discs tissue, l(3)mbt ChIP Canton S 25C third instar larval brain and wing/haltere/third leg imaginal discs
l(3)mbt ChIP, w1118
Drosophila melanogaster GSM722523: l(3)mbt ChIP, w1118 ChIP-Seq Illumina Genome Analyzer II guinea pig 1 anti-l(3)mbt 1244-1477aa (glycine elution) Third instar larval brain and wing/haltere/third leg imaginal discs tissue, l(3)mbt ChIP w1118 25C third instar larval brain and wing/haltere/third leg imaginal discs