A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.

Polarization of the T cell microtubule-organizing center (MTOC) toward the antigen-presenting cell (APC) is driven by the accumulation of diacylglycerol (DAG) at the immunological synapse (IS). The mechanisms that couple DAG to the MTOC are not known. By single-cell photoactivation of the T cell antigen receptor (TCR), we found that three distinct isoforms of protein kinase C were recruited by DAG to the IS in two steps. and accumulated first in a broad region of membrane, whereas arrived later in a smaller zone. Functional experiments indicated that duanyu1531-θ was required for MTOC reorientation and that duanyu1531-ɛ and duanyu1531-η operated redundantly to promote the recruitment of duanyu1531-θ and subsequent polarization responses. Our results establish a previously uncharacterized role for proteins in T cell polarity.

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