PNAS4 knockout does not induce obviously neurocytes apoptosis and abnormal development in mice brain.

Apoptosis is one kind of programmed cell death and contributes to development of a variety of organs such as brain. has been reported to be a novel apoptosis-related gene. Overexpression and knocking down of duanyu1535S4 would cause zebrafish and Xenopus lavis developmental abnormalities. But its function and apoptotic mechanism in mammals are still unknown. Here, we first reported that established duanyu1535S4 CKO (conditional knock out) mice using recombineering technology. We prepared its polyclonal antibodies which recognized both overexpression protein and WT and CKO mice brain tissue and MEFS cells with high titre and specificity. Further we detected that duanyu1535S4 was highly expressed in the embryonic period. However, we observed neither neural structural abnormality nor apoptosis signal in duanyu1535S4 CKO mice brain. Our data suggested that duanyu1535S4 was not involved in mice brain development and apoptosis.

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