Efficient extension of slipped DNA intermediates by DinB is required to escape primer template realignment by DnaQ.

We show that Escherichia coli DinB polymerase, which creates single-base deletions, prefers to extend slipped DNA substrates with the skipped base at the -4 position. A DinB(Y79L) variant, which extends these substrates less efficiently in vitro, allows the proofreading function of polymerase III to reverse their formation in vivo.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}