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[Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants].

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 Feb;19(1):57-9
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摘要

AIM:To investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum. METHODS:We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation. RESULTS:(1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity. CONCLUSION:17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.

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