[Role of TNF-alpha in vascular endothelial cells injury mediated by frozen/thawed PMN].

AIM:To investigate the role of TNF-alpha in vascular endothelial cells injury mediated by freezing/thaw ing cell model was founded using rat isolated by dextran sedimentation technique and VEC cultured in vitro. The injury level of VEC was indicated by measuring activity of LDH in medium. The number of frozen/thawed duanyu1451 adhering to VEC was counted with Phagocytizing reactive dyes the degree of frozen/thawed duanyu1451 and VEC adhesion. Expression of LFA-1 on the surface of frozen/thawed duanyu1451 was analyzed with flow cytometry. RESULTS:TNF-alpha could obviously upregulate expression of LFA-1 on surfaced of frozen/thawed Upregulation of LFA-1 expression promoted adhesion of frozen/thawed duanyu1451 and normal VEC,and aggravated VEC injury. Monoclonal antibody against LFA-1 could partly block adhesion of frozen/thawed duanyu1451 and normal VEC,and attenuate VEC injury. CONCLUSION:TNF-alpha can promote expression of LFA-1 on surface of frozen/thawed duanyu1451 adhering of frozen/thawed duanyu1451 to normal VEC and VEC injury increase, monoclonal antibody against LFA-1 could partly block adhesion and attenuate VEC injury.

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