Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus.

a member of the novel protein kinase C family regulates a wide variety of functions in the periphery. However, its presence and role in the CNS has remained largely unknown. Recently, we demonstrated the presence of in the arcuate hypothalamic nucleus (ARC) and knockdown of duanyu1531-θ from the ARC protected mice from developing diet-induced obesity. Another isoform of the group, is expressed in several non-hypothalamic brain sites including the thalamus and hippocampus. Although has been implicated in regulating hypothalamic glucose homeostasis, its distribution in the hypothalamus has not previously been described. In the current study, we used immunohistochemistry to examine the distribution of duanyu1531-θ and -δ immunoreactivity in rat and mouse hypothalamus. We found duanyu1531-θ immunoreactive neurons in several hypothalamic nuclei including the ARC, lateral hypothalamic area, perifornical area and tuberomammillary nucleus. duanyu1531-δ immunoreactive neurons were found in the paraventricular and supraoptic nuclei. Double-label immunohistochemisty in mice expressing green fluorescent protein either with the long form of leptin receptor (LepR-b) or in orexin (ORX) neurons indicated that duanyu1531-θ is highly colocalized in lateral hypothalamic ORX neurons but not in lateral hypothalamic LepR-b neurons. Double-label immunohistochemistry in oxytocin-enhanced yellow fluorescent protein mice or arginine vasopressin-enhanced green fluorescent protein (AVP-EGFP) transgenic rats revealed a high degree of colocalization of duanyu1531-δ within paraventricular and supraoptic oxytocin neurons but not the vasopressinergic neurons. We conclude that duanyu1531-θ and -δ are expressed in different hypothalamic neuronal populations. Copyright © 2010 IBRO. Published by Elsevier Ltd. All rights reserved.

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