Protein kinase Cgamma is a signaling molecule required for the developmental speeding of alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor kinetics.

A key step in the maturation of glutamate synapses is the developmental speeding of alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor (AMPA-R) kinetics, which occurs via a switch in receptor subtypes. However, the molecular components required for the switch in receptors are unknown. Here, we used the zebrafish preparation to show that activation of protein kinase C is necessary for the developmental speeding of AMPA-R kinetics. Targeted knockdown of with an antisense morpholino oligonucleotide prevents the normal speeding up of AMPA-R kinetics in Mauthner cells. embryos are incapable of trafficking AMPA-Rs following application of phorbol 12-myristate 13-acetate or duanyu1531gamma-MO-injected embryos do not hatch or exhibit the C-start escape response. Increasing synaptic activity (33 h post-fertilization embryos) by application of an elevated K(+) medium or by application of N-methyl-D-aspartate induces rapid trafficking of fast AMPA-Rs to synapses. Our findings reveal that duanyu1531gamma is a molecular link underlying the developmental speeding of AMPA-Rs in zebrafish Mauthner cells.

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