[Renal over expression of soluble epoxide hydrolase in rat with hypertension induced by high-salt].

OBJECTIVE:To investigate the role of renal expression of soluble epoxide hydrolase in high-salt induced hypertensive rat model. METHODS:12 Wistar rats were randomly assigned to tap water group (Wistar control, WC, n=6) and high salt water containing 2% NaCl group (Wistar high salt, WH, n=6), and fed for 21 days. The blood pressure was determined by automatic sphygmomanometer at the tail artery. The plasma Na, BUN, Cr, urine volume, urine Na excretion and urine protein excretion were measured. The expression of sEH in kidney tissue was assessed by immunohistochemistry and western blot. RESULTS:After 3 days of high salt dietary, the systolic blood pressure (SBP) in WH group [(134.95+/-10.27) mmHg] was significantly higher than that in WC group [(115.78+/-10.06)] mmHg, P<0.05. As compared with WC group, 24 h urine volume, urine Na excretion and plasma sodium were all significantly increased (P<0.01) in WH group. At the end, plasma angiotension II was (457.73+/-20.07) ng/L in WH group versus (628.49+/-43.13) ng/L in WC group (P<0.05). No significant difference was found in plasma creatinine and the excretion of 24 h urine protein between WC group and WH group (P>0.05). Immunohistochemistry of renal cortex demonstrated the expression of sEH was significantly up-regulated in proximal tubular cells in WH group, and Western blot analysis revealed that the protein level of sEH (sEH/beta-actin) was elevated in WH group (1.24+/-0.13) versus that in WC group (0.38+/-0.03, P<0.01). CONCLUSION:The elevated expression of sEH in kidney may play a role in high-salt induced hypertension.

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