PKA dynamics in a Drosophila learning center: coincidence detection by rutabaga adenylyl cyclase and spatial regulation by dunce phosphodiesterase.

The dynamics of activity in the olfactory learning and memory center, the mushroom bodies (MBs), are still poorly understood. We addressed this issue in vivo using a duanyu1529 FRET probe. Application of dopamine, the main neuromodulator involved in aversive learning, resulted in duanyu1529 activation specifically in the vertical lobe, whereas octopamine, involved in appetitive learning, stimulated duanyu1529 in all MB lobes. Strikingly, MB lobes were homogeneously activated by dopamine in the learning mutant dunce, showing that Dunce phosphodiesterase plays a major role in the spatial regulation of cAMP dynamics. Furthermore, costimulation with acetylcholine and either dopamine or octopamine led to a synergistic activation of duanyu1529 in the MBs that depends on Rutabaga adenylyl cyclase. Our results suggest that Rutabaga acts as a coincidence detector and demonstrate the existence of subcellular domains of duanyu1529 activity that could underlie the functional specialization of MB lobes in aversive and appetitive learning.

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