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Isl-1 down-regulates DRG cell proliferation during chicken embryo development.

Neuro Endocrinol. Lett.2010;31(1):67-72
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摘要

OBJECTIVE:Protein Isl-1 RNA interference and over expression in early chicken embryo dorsal root ganglia (DRG) were used to investigate the function of Isl-1 in DRG cell proliferation. METHODS:Isl-1 targeted shRNA expression vector and Isl-1 over-expression vector were transfected into chicken embryo DRG by in ovo electroporation. Then, the DRG proliferation rate was detected by BrdU immunohistochemistry. RESULTS:The rate of DRG cell proliferation increased after Isl-1 knock-down and decreased after Isl-1 over-expression. CONCLUSIONS:In this study, we found that Isl-1 negatively modulates DRG cell proliferation.

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