Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.

J. Microbiol. Methods. 2010 Feb;80(2):219-21. Epub 2009 Dec 21

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摘要

To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems.

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Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.

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