[Effect of leptin on inducible NO and MMP-13 in rabbit articular chondrocytes in vitro].

OBJECTIVE:To observe the in vitro effect of leptin, alone or in combination with tumor necrosis factor-alpha (TNF-alpha) on inducible nitric oxide(NO)and on inducible matrix metalloproteinase-13 (MMP-13) in rabbit articular chondrocytes. METHODS:The chondrocytes from the articular cartilage of 2-month-old rabbits were cultivated and identified, and the second filial generation chondrocytes were cocultured on plates with different concentrations of leptin alone or in combination with TNF-alpha for 48 h or 96 h after 12 h starvation. The concentration of NO and MMP-13 was measured in the chondrocytes culture supernatant fluid. The results were statistically analyzed. RESULTS:There was no significant difference in the concentrations of NO between the different concentrations of leptin alone groups and the blank control group (P>0.05). In combination with the same concentration of TNF-alpha (10 ng/mL), leptin could dose-dependently increase the concentration of NO in the chondrocytes culture supernatant fluid in vitro. There was significant value in average concentration of MMP-13 on the main effect of both time and dose (P<0.05). No MMP-13 was detected in the blank control group. CONCLUSION:Leptin can induce MMP-13 and have synergistic induction effect on NO with TNF-alpha in rabbit articular chondrocytes in vitro.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}