Expression of the Arabidopsis thaliana histone gene AtHTA1 enhances rice transformation efficiency.

Mol Plant. 2009 Jul;2(4):832-837. Epub 2009 Jun 15

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We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.

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Expression of the Arabidopsis thaliana histone gene AtHTA1 enhances rice transformation efficiency.

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