IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster.
Biochemistry. 2009 Nov 10;48(44):10483-5. doi:10.1021/bi901519q
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摘要
IspG is a [4Fe-4S] cluster-containing protein, and the [4Fe-4S](+) species is proposed to be the catalytically relevant species. However, attempts reported in the literature failed to detect the [4Fe-4S](+) species. In this study, using a potent reduction system, we have successfully detected the [4Fe-4S](+) species with X-band EPR spectroscopy. In addition, we have improved the Escherichia coli IspG activity to 550 nmol min(-1) mg(-1), which is approximately 20-fold greater than that of the NADPH-Fpr-FldA system in the literature.
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基因功能
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原始数据
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文献解读
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